Resonant Scanning
Resonant Scanning - Related Videos
08:01
Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
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Cited by 11 •
2016
A new electron paramagnetic resonance (EPR) method, rapid scan EPR (RS-EPR), is demonstrated for 2D spectral spatial imaging which is superior to the traditional continuous wave (CW) technique and opens new venues for in vivo imaging. Results are demonstrated at 250 MHz, but the technique is applicable at any frequency.
02:52
Resonance
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2020
The Lewis structure of a nitrite anion (NO2−) may actually be drawn in two different ways, distinguished by the locations of the N-O and N=O bonds. If nitrite ions do indeed contain a single and a double bond, the two bond lengths are expected to be different. A double bond between two atoms is shorter (and stronger) than a single bond between the same two atoms. However, experiments show that both N–O bonds in NO2− have the same strength and length, and are identical in all other...
02:28
Leaky Scanning
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2020
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
10:26
Fabrication and Characterization of Superconducting Resonators
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Cited by 3 •
2016
Superconducting microwave resonators are of interest for detection of light, quantum computing applications and materials characterization. This work presents a detailed procedure for fabrication and characterization of superconducting microwave resonator scattering parameters.
10:28
Fabrication of Nanopillar-Based Split Ring Resonators for Displacement Current Mediated Resonances in Terahertz Metamaterials
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Cited by 1 •
2017
A protocol for the design and fabrication of a novel nanopillar-based split ring resonator (SRR) is presented.
FAQs
The mirror’s natural resonant frequency provides a stable high-speed oscillation for scanning. Driving it at that frequency makes the focused laser beam sweep repeatedly across the specimen, allowing image acquisition to keep pace with biological changes. This rapid temporal sampling is especially useful when movement or activity could substantially alter the specimen during image collection.
As the focused laser traverses the specimen, biological structures emit fluorescence that the system detects. The detected signal is converted into image data and associated with the beam’s scanning position, allowing the image to represent spatial fluorescence patterns. Repeated sweeps support rapid collection while preserving information about changing cellular or tissue features.
Rapid acquisition shortens the interval needed to collect an image, reducing the opportunity for moving structures or signals to shift during scanning. This can help limit motion blur in dynamic specimens. It can also reduce the time a living sample remains exposed to illumination, an important consideration when observing biological processes over time.
The process begins by driving the scanning mirror at its resonant frequency and directing the focused laser across the specimen. Emitted fluorescence is then detected during the sweep and converted into image data. Repeating this sequence produces rapidly acquired images that can track biological systems whose structures or signals change over time.
Resonant scanning is used in fluorescence microscopy and multiphoton microscopy. In both settings, its rapid image acquisition supports observation of biological activity that changes quickly. The approach is therefore relevant when researchers need to follow cellular or tissue events over time while limiting motion-related image changes and the duration of illumination exposure.
Applications include studying cell behavior, neuronal activity, and intracellular dynamics, along with other biological processes that change quickly. The method provides time-resolved fluorescence image data, helping researchers observe how structures or signals evolve rather than relying only on static snapshots. Its value is greatest when biological changes occur during the imaging period.