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JoVE Journal
Biology

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Generation of Myospheres From hESCs by Epigenetic Reprogramming
 

Generation of Myospheres From hESCs by Epigenetic Reprogramming

Article DOI: 10.3791/51243
June 21st, 2014

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Summary June 21st, 2014

Here, we describe a protocol based on epigenetic reprogramming of human embryonic stem cells (hESCs) toward generating a homogeneous population of skeletal muscle progenitors that under permissive culture conditions form three-dimensional clusters of contractile myofibers (myospheres), which recapitulate biological features of human skeletal muscles.

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