Biology
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Generation of Myospheres From hESCs by Epigenetic Reprogramming
Chapters
Summary June 21st, 2014
Here, we describe a protocol based on epigenetic reprogramming of human embryonic stem cells (hESCs) toward generating a homogeneous population of skeletal muscle progenitors that under permissive culture conditions form three-dimensional clusters of contractile myofibers (myospheres), which recapitulate biological features of human skeletal muscles.
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