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Generation of Myospheres From hESCs by Epigenetic Reprogramming
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Biology
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JoVE Journal Biology
Generation of Myospheres From hESCs by Epigenetic Reprogramming

Generation of Myospheres From hESCs by Epigenetic Reprogramming

DOI:
10.3791/51243-v

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09:32 min

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June 21, 2014

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,
1Muscle Development and Regeneration Program,Sanford-Burnham Institute for Medical Research, 2IRCCS Fondazione Santa Lucia

Chapters

  • 00:05Title
  • 01:23Infection Procedure
  • 03:46Culturing the Infected Cells
  • 05:35EB-like Induction and Differentiation
  • 07:33Representative Results: Myogenic Differentiation of Human Embryonic Stem Cells
  • 08:56Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a protocol based on epigenetic reprogramming of human embryonic stem cells (hESCs) toward generating a homogeneous population of skeletal muscle progenitors that under permissive culture conditions form three-dimensional clusters of contractile myofibers (myospheres), which recapitulate biological features of human skeletal muscles.

Tags

Keywords: HESCsSkeletal Muscle CellsMyospheresEpigenetic ReprogrammingMyoDBAF60CNeuromuscular Disorders3D Contractile StructuresStem Cell DifferentiationMiniaturized Skeletal Muscles

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