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Generation of Myospheres From hESCs by Epigenetic Reprogramming
JoVE Journal
Biology
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JoVE Journal Biology
Generation of Myospheres From hESCs by Epigenetic Reprogramming

Generation of Myospheres From hESCs by Epigenetic Reprogramming

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09:32 min

June 21, 2014

DOI:

09:32 min
June 21, 2014

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Here, we describe a protocol based on epigenetic reprogramming of human embryonic stem cells (hESCs) toward generating a homogeneous population of skeletal muscle progenitors that under permissive culture conditions form three-dimensional clusters of contractile myofibers (myospheres), which recapitulate biological features of human skeletal muscles.

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