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June 21, 2014
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Here, we describe a protocol based on epigenetic reprogramming of human embryonic stem cells (hESCs) toward generating a homogeneous population of skeletal muscle progenitors that under permissive culture conditions form three-dimensional clusters of contractile myofibers (myospheres), which recapitulate biological features of human skeletal muscles.
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Cite this Article
Albini, S., Puri, P. L. Generation of Myospheres From hESCs by Epigenetic Reprogramming. J. Vis. Exp. (88), e51243, doi:10.3791/51243 (2014).
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