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手术应激探索术后免疫抑制的机制和评估新围手术期免疫疗法的小鼠肿瘤模型
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
A Mouse Tumor Model of Surgical Stress to Explore the Mechanisms of Postoperative Immunosuppression and Evaluate Novel Perioperative Immunotherapies
Please note that all translations are automatically generated.
Click here for the English version.
手术应激探索术后免疫抑制的机制和评估新围手术期免疫疗法的小鼠肿瘤模型
DOI:
10.3791/51253-v
•
13:37 min
•
March 12, 2014
•
Lee-Hwa Tai
,
Christiano Tanese de Souza
,
Shalini Sahi
,
Jiqing Zhang
2,4
,
Almohanad A Alkayyal
3,5
,
Abhirami Anu Ananth
3
,
Rebecca A.C. Auer
6
1
Centre for Innovative Cancer Research
,
Ottawa Hospital Research Institute
,
2
Department of Cellular and Molecular Medicine
,
University of Ottawa
,
3
Department of Biochemistry, Microbiology and Immunology
,
University of Ottawa
,
4
Department of Neurosurgery
,
The Second Hospital of Shandong University
,
5
Department of Medical Laboratory Technology
,
University of Tabuk
,
6
Department of Surgery
,
Ottawa General Hospital
Chapters
00:05
Title
01:41
Culturing and Harvesting 4T1 Tumor Cells
03:18
Injecting Mice with 4T1 Tumor Cells
04:39
Administrating Perioperative Treatment in 4T1 Tumor Bearing BALB/c Mice
06:03
Complete Resection of Primary Tumor and Abdominal Left Nephrectomy
08:23
Lung Removal and Quantification of Lung Tumor Burden
09:32
Results: Lung Metastases, Surgical Stress, and Perioperative Therapy
12:53
Conclusion
Summary
Automatic Translation
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Automatic Translation
手术应激的小鼠肿瘤模型来探讨术后免疫抑制如何促进转移性疾病,并评价免疫围手术期治疗。
Tags
Mouse Tumor Model
Surgical Stress
Postoperative Immunosuppression
Perioperative Immunotherapies
Cancer Surgery
Metastatic Disease
NK Cells
4T1 Breast Cancer
Lung Metastases
Immunotherapy
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