Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

Miquella G. Chavez; Jimmy Hu; Kerstin Seidel; Chunying Li; Andrew Jheon; Adrien Naveau; Orapin Horst; Ophir D. Klein

doi:10.3791/51266

Journal

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Biology

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Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

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Biology

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JoVE Journal Biology

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

15,052 Views

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08:14 min

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May 01, 2014

DOI:

10.3791/51266-v

DOI:

10.3791/51266-v

•

08:14 min

May 01, 2014

•

4 Views

Miquella G. Chavez^1,2, Jimmy Hu¹, Kerstin Seidel¹, Chunying Li^1,3, Andrew Jheon¹, Adrien Naveau^4,5,6, Orapin Horst^1,7, Ophir D. Klein^1,8

¹Department of Orofacial Sciences and Program in Craniofacial and Mesenchymal Biology,University of California, San Francisco, ²Department of Bioengineering and Therapeutic Sciences,University of California, San Francisco, ³Department of Pathology and Research Center,Zhongshan Hospital of Dalian University, ⁴Université Paris Descartes, Sorbonne Paris Cite, UMR S872, ⁵Centre de Recherche des Cordeliers,Université Pierre et Marie Curie, UMR S872, ⁶INSERM U872, ⁷Division of Endodontics, Department of Preventive and Restorative Dental Sciences,University of California, San Francisco, ⁸Department of Pediatrics and Institute for Human Genetics,University of California, San Francisco

Summary

The continuously growing mouse incisor provides a model for studying renewal of dental tissues from dental epithelial stem cells (DESCs). A robust system for consistently and reliably obtaining these cells from the incisor and expanding them in vitro is reported here.