Deep Brain Stimulation with Simultaneous fMRI in Rodents

John Robert Younce; Daniel L Albaugh; Yen-Yu Ian Shih

doi:10.3791/51271

Deep Brain Stimulation with Simultaneous fMRI in Rodents

JoVE Journal
Neuroscience
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JoVE Journal Neuroscience

Deep Brain Stimulation with Simultaneous fMRI in Rodents

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11:09 min

February 15, 2014

DOI: 10.3791/51271-v

John Robert Younce^1,2,5, Daniel L Albaugh^1,2,4, Yen-Yu Ian Shih^1,2,3,4

¹Department of Neurology,University of North Carolina, ²Biomedical Research Imaging Center,University of North Carolina, ³Department of Biomedical Engineering,University of North Carolina, ⁴Curriculum in Neurobiology,University of North Carolina, ⁵School of Medicine,University of North Carolina

Overview

This protocol describes a method for simultaneous functional magnetic resonance imaging and deep brain stimulation in rodents. This technique enables the mapping of brain responses to electrical stimulation across various targets.

Key Study Components

Area of Science

Neuroscience
Neuroimaging
Electrophysiology

Background

Functional MRI provides insights into brain activity.
Deep brain stimulation is used to modulate neuronal activity.
Combining these techniques allows for comprehensive analysis.
This method is applicable in rodent models for research.

Purpose of Study

To explore global brain activity in response to stimulation.
To assess the effects of deep brain stimulation at various targets.
To enhance understanding of brain function and connectivity.

Methods Used

Stereotactic electrode implantation
Functional MRI acquisition
Analysis of functional responses
Mapping of brain activity

Main Results

Identification of brain regions activated by stimulation.
Characterization of functional connectivity changes.
Insights into the dynamics of brain response patterns.
Potential applications in understanding neurological disorders.

Conclusions

This method provides a powerful tool for neuroscience research.
It allows for the investigation of brain function in real-time.
Future studies can build on this approach for deeper insights.

Frequently Asked Questions

What is the significance of combining fMRI and deep brain stimulation?

This combination allows researchers to observe real-time brain activity in response to targeted electrical stimulation, enhancing our understanding of brain function.

What animal model is used in this study?

The study utilizes rodent models, specifically rats, for the experimental procedures.

What are the main steps involved in the protocol?

The main steps include electrode implantation, fMRI acquisition, and analysis of functional responses.

How does this method contribute to neuroscience?

It provides insights into brain connectivity and the effects of stimulation, which can inform treatments for neurological conditions.

Can this technique be applied to other animal models?

While this protocol focuses on rodents, similar techniques may be adapted for other species in future research.

What are the potential applications of this research?

The findings could have implications for understanding and treating various neurological disorders through targeted stimulation.

This protocol describes a standard method for simultaneous functional magnetic resonance imaging and deep brain stimulation in the rodent. The combined use of these experimental tools allows for the exploration of global downstream activity in response to electrical stimulation at virtually any brain target.

Hi, I'm John Ya.And I'm Dan Alba. Today we'll demonstrate functional MRI will. Simultaneous deep brain stimulation in a rat.

We can use this technique to map functional responses throughout the brain to deep brain stimulation at a variety of targets. This procedure involves stereotactic electrode implantation FMRI acquisition, an analysis of functional responses. The first step is electrode implantation.

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