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Myelin Oligodendrocyte Glycoprotein (MOG35-55) Induced Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6 Mice
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Myelin Oligodendrocyte Glycoprotein (MOG35-55) Induced Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6 Mice

Myelin Oligodendrocyte Glycoprotein (MOG35-55) Induced Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6 Mice

DOI:
10.3791/51275-v

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08:03 min

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April 15, 2014

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, , ,
1Department of Neurology,University of Münster, 2Interdisciplinary Center for Clinical Research (IZKF), Münster, 3Institute of Physiology – Neuropathophysiology,University of Münster

Chapters

  • 00:05Title
  • 01:51Preparation of MOG35-55 Emulsion
  • 04:19Preparation of Pertussis Toxin
  • 05:03Animal Immunization
  • 06:14EAE Monitoring
  • 07:04Results: EAE Symptoms After Immunization
  • 07:29Conclusion

Summary

Automatic Translation

Automatic Translation

Experimental autoimmune encephalomyelitis (EAE) is an established animal model of multiple sclerosis. C57BL/6 mice are immunized with myelin oligodendrocyte glycoprotein (MOG) peptide 35-55 (MOG35-55), resulting in an ascending flaccid paralysis caused by autoreactive immune cells in the central nervous system. Protocols for disease induction and monitoring will be discussed.

Tags

Keywords: Experimental Autoimmune EncephalomyelitisEAEMultiple SclerosisMOG35-55C57BL/6 MiceNeuroinflammationDemyelinationAutoimmunityAnimal Model

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