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Immunology and Infection

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JoVE Journal Immunology and Infection
Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Journal (Immunology and Infection)
High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry
Journal (Immunology and Infection)
Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry
 

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Article DOI: 10.3791/51284 • 09:04 min
May 22nd, 2014 •
1, 2, 3
1Department of Pathology, New York University School of Medicine, 2Division of Infectious Diseases, Department of Medicine, Mount Sinai Medical Center, 3Division of Hematology and Oncology, Hess Center for Science and Medicine, Mount Sinai Medical Center

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Summary
Automatic Translation
May 22nd, 2014

Dendritic cells (DCs) secrete IL-1β in response to TLR8 recognition of synthetic purine, R848, followed by NLRP3 inflammasome activation with nigericin, therefore, IL-1β can be used to measure NLRP3 inflammasome activity. Intracellular cytokine staining, immunoblotting, and ELISA are used to accurately measure NLRP3 inflammasome priming and activation via IL-1β expression.

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