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测定蛋白酶体降解的植物无细胞系统
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JoVE Journal Biology
Assaying Proteasomal Degradation in a Cell-free System in Plants
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测定蛋白酶体降解的植物无细胞系统

DOI:
10.3791/51293-v

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07:43 min

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March 26, 2014

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, ,
1Department of Biochemistry and Cell Biology,Stony Brook University, State University of New York

Chapters

  • 00:05Title
  • 01:31Plant Growth and Inoculation with Agrobacterium
  • 03:09Preparation of Cell-free Extracts
  • 04:08Detecting Protein Degradation by Immunoblotting
  • 06:01Results: VBF Promotes Proteasomal Degradation of VIP1
  • 07:07Conclusion

Summary

Automatic Translation

Automatic Translation

有针对性的蛋白质降解是一个重大的监管机制，细胞的功能。它发生通过一个保守的泛素-蛋白酶体通路，其高度多聚泛素链的靶蛋白，然后作为分子的“标签”为26S蛋白酶体。在这里，我们描述了用于蛋白质的蛋白酶体降解的简单和可靠的无细胞测定法。

Tags

Keywords: Ubiquitin-proteasome PathwayProtein DegradationPlantsUbiquitin/26S Proteasome System (UPS)E3 Ubiquitin LigaseSCF ComplexF-box ProteinCell-free SystemProtein Degradation Assay

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