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Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
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Bioengineering
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JoVE Journal Bioengineering
Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration

Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration

DOI:
10.3791/51295-v

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12:03 min

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January 22, 2014

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, , ,
1Department of Chemical and Biomolecular Engineering,University of Akron

Chapters

  • 00:05Title
  • 02:55Bacterial Cell Culture
  • 04:25Isolation and Purification of Recombinant Protein
  • 05:42Ni-NTA Affinity Chromatography
  • 08:31Results: Purification of Native and Nonnative Proteins for Biotinylation
  • 10:32Conclusion

Summary

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Developing biotinylatable fusion proteins has many potential applications in various fields of research. Recombinant protein engineering is a straight forward procedure that is cost-effective, providing high yields of custom-designed proteins.

Tags

Recombinant Protein EngineeringEscherichia ColiBiotin TagStreptavidinGreen Fluorescent ProteinUnnatural Amino AcidsMetal Ion ChelatorsPost-translational ModificationsFusion ProteinsBiotinylatable ProteinNerve Growth FactorSemaphorin3ABiomaterialsRegenerative MedicineT7 Lac Inducible Vector

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