JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Neuroscience
/
有丝分裂中的不同发育阶段小鼠胚胎皮层实时成像
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Neuroscience
Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex
Please note that all translations are automatically generated.
Click here for the English version.
有丝分裂中的不同发育阶段小鼠胚胎皮层实时成像
DOI:
10.3791/51298-v
•
09:25 min
•
June 04, 2014
•
Louis-Jan Pilaz
,
Debra L. Silver
2
1
Department of Molecular Genetics and Microbiology
,
Duke University Medical Center
,
2
Departments of Neurobiology and Cell Biology, Duke Institute for Brain Sciences
,
Duke University Medical Center
Chapters
00:05
Title
01:17
Dissection and Embedding of the Embryonic Brains
02:24
Embedded Brain Sectioning and Staining
03:59
Live Imaging of the Slices
04:55
Post-acquisition Analysis of Mitosis
07:34
Results: Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex
08:43
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
神经祖细胞有丝分裂是神经发生的一个关键参数。很多我们的神经祖有丝分裂的理解是基于固定的组织分析。在胚胎大脑切片实时成像是一种通用的技术,以评估有丝分裂与在受控环境中高时空分辨率。
Tags
Mitosis
Live Imaging
Mouse Embryonic Cortex
Neural Progenitors
Brain Development
Cerebral Cortex
Time-lapse Imaging
Syto11
Histone H2B-EGFP
Centrin-EGFP
MCherry-α-tubulin
Genetic Regulation
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
慢性成像小鼠的视觉皮层使用减薄,颅骨的制备
多鼠神经解剖学磁共振成像
在发展中国家斑马鱼的前脑的神经祖细胞克隆相关的定时实时成像
高分辨率实时成像发展中的神经上皮细胞的行为
体外
实时成像在小鼠神经上皮单细胞分裂
双光子<em>在体内</em>树突棘中的鼠标皮质用稀疏的头颅成像准备
室管膜纤毛在小鼠脑侧脑室实时成像
同时双光子<em>在体内</em>突触输入的影像和突触后目标中的鼠标Retrosplenial的Cortex
生活成像的脑皮层细胞使用二维培养系统
前子宫
内胚胎小鼠脑电穿孔和脊髓切片培养迁移 GABAergic 中间神经元的活体成像
Read Article