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Neuroscience

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Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model
 

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model

Article DOI: 10.3791/51305
April 16th, 2014

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Summary April 16th, 2014

Multiplex assays can provide beneficial information for basic cellular mechanisms and eliminate waste of reagents and unnecessary repetitive experiments. We describe here a multiplex caspase-3/7 activity assay, using fluorescent- and luminescent-based methods, to determine cell viability in an in vitro hypothalamic model following oxidative challenge with palmitic acid.

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