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JoVE Journal Biology
Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis
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Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis

Article DOI: 10.3791/51312
September 1st, 2014 •
1, 1, 2, 1, 1, 1, 1, 1, 1, 1, 2
1Department of Biology, American University, 2Laboratory of Cancer Biology and Genetics, National Cancer Institute, NIH

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September 1st, 2014

The tube formation assay is a fast, quantifiable method for measuring in vitro angiogenesis. Endothelial cells are combined with conditioned media and plated on basement membrane extract. Tube formation occurs within hours and newly formed tubules easily quantified.

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