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Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis
JoVE Journal
Biology
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JoVE Journal Biology
Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis
DOI:

08:12 min

September 01, 2014

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Chapters

  • 00:05Title
  • 01:29Growing and Starving Endothelial Cells
  • 02:07Preparing Reagents
  • 02:37Preparing Endothelial Cells
  • 03:35Tube Formation Assay
  • 05:59Results: Endothelial Tube Formation
  • 07:46Conclusion

Summary

Automatic Translation

The tube formation assay is a fast, quantifiable method for measuring in vitro angiogenesis. Endothelial cells are combined with conditioned media and plated on basement membrane extract. Tube formation occurs within hours and newly formed tubules easily quantified.

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