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Journal / Neuroscience / A Method to Make a Craniotomy on the Ventral…
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Neuroscience

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JoVE Journal Neuroscience
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A Method to Make a Craniotomy on the Ventral Skull of Neonate Rodents

Article DOI: 10.3791/51350 • 08:30 min • May 22nd, 2014
May 22nd, 2014 •
1, 2, 2
1Department of Biology, The City University of New York, City College, 2Department of Biomedical Engineering, The City University of New York, City College

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Summary
Automatic Translation

A surgical method is described to expose the ventral skull in neonate rats. Using this approach it is possible to open a craniotomy to perform acute electrophysiology and two-photon microscopy experiments in the brainstem of anesthetized pups.

Tags

Craniotomy Ventral Skull Neonate Rodents In Vivo Experiments Cellular Processes Mammalian Brain Adulthood Development Brain Regions Dorsal Side Brainstem Regions Pons Understudied Protocol Access To Ventral Brainstem Structures Electrophysiological Methods Imaging Methods Structural Changes Long-range Axons Electrical Activity Ensembles Of Cells Blood Brain Barrier Permeability Neonate Animals Auditory Brainstem Neonate Rats Rodent Species
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