A surgical method is described to expose the ventral skull in neonate rats. Using this approach it is possible to open a craniotomy to perform acute electrophysiology and two-photon microscopy experiments in the brainstem of anesthetized pups.
Rodríguez-Contreras, A., Shi, L., Fu, B. M. A Method to Make a Craniotomy on the Ventral Skull of Neonate Rodents. J. Vis. Exp. (87), e51350, doi:10.3791/51350 (2014).