Cell Labeling and Injection in Developing Embryonic Mouse Hearts

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Biology

JoVE Journal
Biology

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JoVE Journal Biology

Cell Labeling and Injection in Developing Embryonic Mouse Hearts

DOI:

10.3791/51356-v

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07:20 min

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April 17, 2014

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Emilye Hiriart*¹, Patrick van Vliet*², Ralf J. Dirschinger, Sylvia M. Evans, Michel Puceat

¹INSERM UMR-910,Aix-Marseille University, ²Skaggs School of Pharmacy and Pharmaceutical Sciences,University of California, San Diego

Chapters

00:05Title
01:49Collection of E9.5 and E10.5 Embryos for Ex vivo Injection
03:10Ex vivo DNA or Cell Injection Under a Stereomicroscope
04:35Results: Embryonic Heart Cell Labeling and Injection
06:30Conclusion

Summary

Automatic Translation

We describe a series of methods to inject dyes, DNA vectors, virus, and cells in order to monitor both cell fate and phenotype of endogenous and grafted cells derived from embryonic or pluripotent cells within mouse embryos at embryonic day (E)9.5 and later stages of development.

Cell Labeling and Injection in Developing Embryonic Mouse Hearts

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Chapters

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