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Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy

Article DOI: 10.3791/51357-v • 06:51 min • May 21st, 2014
May 21st, 2014 •
1, 1, 1, 2, 1
1Department of Biochemistry and Cancer Biology, University of Toledo College of Medicine and Life Sciences, 2Department of Physiology and Pharmacology, University of Toledo College of Medicine and Life Sciences

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Summary
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We describe a reliable method for isolation of adult mouse cardiomyocytes. This protocol yields a consistent result for the culture of functional adult cardiomyocytes from a variety of genetically modified mice.

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Isolation Culture Adult Mouse Cardiomyocytes Cell Signaling In Vitro Cardiac Hypertrophy Genetically Modified Mice Transgenic Mice Gene Knockout Mice Cardiac Cellular Physiology Pathophysiology Pharmaceutical Intervention Cardiomyocyte Infection Processes High Quantity Quality Functional Cardiomyocytes Cardiovascular Research Cell Signaling Transduction Research Drug Development Well-established Method Excised Heart Isolated Heart System Perfusion Calcium-free Buffer High Potassium Buffer Type II Collagenase Digestion Langendorff Retrograde Perfusion Mode
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