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Nucleoside Triphosphates – From Synthesis to Biochemical Characterization
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JoVE Journal Chemistry
Nucleoside Triphosphates – From Synthesis to Biochemical Characterization

Nucleoside Triphosphates – From Synthesis to Biochemical Characterization

DOI:
10.3791/51385-v

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15:22 min

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April 03, 2014

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, ,
1Department of Chemistry and Biochemistry,University of Bern

Chapters

  • 00:05Title
  • 02:30Synthesis of the Modified Nucleoside Triphosphates
  • 05:56HPLC Purification of the Modified Nucleoside Triphosphates
  • 07:40Primer Extension Reactions and TdT Polymerizations
  • 11:05Results: Biochemical Characterization of Nucleoside Triphosphates
  • 14:02Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol described herein aims to explain and abridge the numerous obstacles in the way of the intricate route leading to modified nucleoside triphosphates. Consequently, this protocol facilitates both the synthesis of these activated building-blocks and their availability for practical applications.

Tags

Nucleoside TriphosphatesPhosphoramidite PrecursorsSolid-phase SynthesisModified Nucleic AcidsFunctional TaggingRibozymesDNAzymesPhosphorous(III)-based ReagentsPrimer Extension ReactionsTdT Tailing PolymerizationChemical Biology

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