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JoVE Journal Neuroscience
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Preparation of Neuronal Co-cultures with Single Cell Precision

Article DOI: 10.3791/51389-v • 09:06 min • May 20th, 2014
May 20th, 2014 •
1, 1,2, 1, 1, 1, 1,3
1Leibniz-Institut für Analytische Wissenschaften, ISAS, 2Department of Biochemical Engineering, University College London, 3Institute for Life Sciences, University of Southampton

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Summary
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Protocols for single neuron microfluidic arraying and water masking for the in-chip plasma patterning of biomaterial coatings are described. Highly interconnected co-cultures can be prepared using minimal cell inputs.

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Neuronal Co-cultures Single Cell Precision Microfluidic Embodiments Campenot Chamber Interconnected Co-culture Platforms Developmental Neurobiology Functional Neurobiology Infection And Disease Propagation Low Abundance Cells Primary Dopaminergic Substantia Nigra Spiral Ganglia Drosophila Melanogaster Neurons High Throughput Experimentation Dense Cultures Microfluidic Single Neuron Arraying Method Water Masking Method Plasma Patterning Biomaterial Coatings Intercompartment Connectivity High Throughput Neurobiology Experiments
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