Journal
/
Neuroscience
/
Preparation of Neuronal Co-cultures with Single Cell Precision
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Preparation of Neuronal Co-cultures with Single Cell Precision

Preparation of Neuronal Co-cultures with Single Cell Precision

DOI:
10.3791/51389-v

•

09:06 min

•

May 20, 2014

•

, , , , ,
1Leibniz-Institut für Analytische Wissenschaften,ISAS, 2Department of Biochemical Engineering,University College London, 3Institute for Life Sciences,University of Southampton

Chapters

  • 00:05Title
  • 02:04Microfluidic Device Replication in PDMS
  • 03:26Device Assembly
  • 04:40Water Masking for In situ Biomaterial Patterning by Plasma Stenciling
  • 06:39Microfluidic Single Neuron Arraying and Culture
  • 07:49Selective Fluidic Treatment
  • 08:37Conclusion

Summary

Automatic Translation

Automatic Translation

Protocols for single neuron microfluidic arraying and water masking for the in-chip plasma patterning of biomaterial coatings are described. Highly interconnected co-cultures can be prepared using minimal cell inputs.

Tags

Keywords: MicrofluidicCo-cultureSingle CellCampenot ChamberNeuroscienceNeurobiologyLow Abundance CellsPrimary DopaminergicSubstantia NigraSpiral GangliaDrosophilia MelanogasterHigh ThroughputCell PatterningPlasma PatterningBiomaterial CoatingsOutgrowthConnectivity

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/51389?article_type=v

Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues
check_url/51389?article_type=v

Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry
check_url/51389?article_type=v

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution
check_url/51389?article_type=v

Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures
check_url/51389?article_type=v

A Procedure for Implanting Organized Arrays of Microwires for Single-unit Recordings in Awake, Behaving Animals
check_url/51389?article_type=v

Inducing Plasticity of Astrocytic Receptors by Manipulation of Neuronal Firing Rates
check_url/51389?article_type=v

Investigating Functional Regeneration in Organotypic Spinal Cord Co-cultures Grown on Multi-electrode Arrays
check_url/51389?article_type=v

Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice
check_url/51389?article_type=v

In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity
check_url/51389?article_type=v

Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity

Read Article