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Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
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Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Article DOI: 10.3791/51406 • 09:43 min • April 23rd, 2014
April 23rd, 2014 •
1, 1, 1
1Institute for Cell Engineering, Department of Neurology and Neuroscience, Johns Hopkins University School of Medicine

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Here, we present our established method to reprogram human somatic cells into transgene-free human iPSCs with Sendai virus, which shows consistent outcome and enhanced efficiency.

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Human Induced Pluripotent Stem Cells IPSCs Biomedicine Genetic Diseases Autologous Cell Therapy Gene Correction Personalized Drug Screening Reprogramming Factor Transgene Expression Integration-free IPSCs Transgene-free IPSCs Adenovirus PiggyBac System Minicircle Vector Episomal Vectors Direct Protein Delivery Synthesized MRNA Sendai-virus Reprogramming System
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