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JoVE Journal
Immunology and Infection

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Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE
 

Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE

Article DOI: 10.3791/51415-v 12:43 min July 29th, 2014
July 29th, 2014

Chapters

Summary

Innate defenses to virus infections are triggered by pattern recognition receptors (PRRs). The two cytoplasmic PRRs RIG-I and PKR bind to viral signature RNAs, change conformation, oligomerize, and activate antiviral signaling. Methods are described which allow to conveniently monitor the conformational switching and the oligomerization of these cytoplasmic PRRs.

Tags

Pattern Recognition Receptors RIG-I PKR Antiviral Response Protease Digestion Native PAGE Host Defenses Viral RNA Ligands Interferon Response Gene Expression Trypsin Digestion Protease Resistance Phosphorylation Oligomeric Complexes
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