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JoVE Journal Neuroscience
Lineage-reprogramación de células derivadas de pericitos del cerebro humano adulto en neuronas inducidas
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Lineage-reprogramación de células derivadas de pericitos del cerebro humano adulto en neuronas inducidas

Article DOI: 10.3791/51433-v • 09:36 min • May 12th, 2014
May 12th, 2014 •
1, 2, 3, 1,4
1Department of Physiological Genomics, Institute of Physiology, Ludwig Maximilians University Munich, 2Tumor Biology Lab, Neurosurgical Clinic, Ludwig-Maximilians University Munich, 3Institut für Biochemie, Emil-Fischer-Zentrum, Friedrich-Alexander-Universität Erlangen-Nürnberg, 4Institute of Physiological Chemistry and Focus Program Translational Neuroscience, Johannes Gutenberg University Mainz

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Atacar a las células cerebrales residente por linaje reprogramación directa ofrece nuevas perspectivas para la reparación del cerebro. Aquí se describe un protocolo de cómo preparar cultivos enriquecidos por pericitos cerebrales residente del adulto corteza cerebral humana y convertirlas en neuronas inducida por la expresión mediada por retrovirus de los factores de transcripción Sox2 y Ascl1.

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Neurociencia Número 87 pericitos linaje reprogramación las neuronas inducidos la corteza cerebral
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