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Lineage-omprogrammering af pericyt-afledte Celler af Voksen Menneskelige hjerne til Inducerede Neuroner
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1Department of Physiological Genomics, Institute of Physiology,Ludwig Maximilians University Munich, 2Tumor Biology Lab, Neurosurgical Clinic,Ludwig-Maximilians University Munich, 3Institut für Biochemie, Emil-Fischer-Zentrum,Friedrich-Alexander-Universität Erlangen-Nürnberg, 4Institute of Physiological Chemistry and Focus Program Translational Neuroscience,Johannes Gutenberg University Mainz
Chapters
- 00:05Title
- 01:34Isolation and Culturing of Adult Human Brain Cells
- 02:55In vitro Expansion and Characterization of Adult Human Brain Cells
- 05:15Retroviral Transduction of Pericyte-derived Cells and Reprogramming into Induced Neurons
- 06:41Results: Reprogramming Adult Brain Cells into Induced Neurons
- 09:11Conclusion
Målretning hjerne-resident celler til direkte afstamning-omprogrammering giver nye perspektiver for hjernen reparation. Her beskriver vi en protokol om, hvordan man forbereder kulturer beriget med hjerne-resident pericytes fra voksent menneske hjernebarken og konvertere disse til inducerede neuroner ved retrovirusmedieret udtryk for transskriptionsfaktorerne Sox2 og Ascl1.
