Isolation of Small Noncoding RNAs from Human Serum

Samantha Khoury; Pamela Ajuyah; Nham Tran

doi:10.3791/51443

Journal

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Biology

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Isolation of Small Noncoding RNAs from Human Serum

JoVE Journal
Biology

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JoVE Journal Biology

Isolation of Small Noncoding RNAs from Human Serum

18,033 Views

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06:44 min

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June 19, 2014

DOI:

10.3791/51443-v

DOI:

10.3791/51443-v

•

06:44 min

June 19, 2014

•

3 Views

Samantha Khoury¹, Pamela Ajuyah¹, Nham Tran^2,3

¹School of Medical and Molecular Biosciences, Faculty of Science,University of Technology, Sydney, ²Centre for Health Technologies, Faculty of Engineering and Information Technology,University of Technology, Sydney, ³The Sydney Head and Neck Cancer Institute, Sydney Cancer Centre,Royal Prince Alfred Hospital

Summary

This protocol describes a method for extracting small RNAs from human serum. We have used this method to isolate microRNAs from cancer serum for use in DNA arrays and also singleplex quantitative PCR. The protocol utilizes phenol and guanidinium thiocyanate reagents with modifications to yield high quality RNA.