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JoVE Journal Biology
Isolation of Small Noncoding RNAs from Human Serum
 

Isolation of Small Noncoding RNAs from Human Serum

Article doi: 10.3791/51443
June 19th, 2014 •
1, 1, 2,3
1School of Medical and Molecular Biosciences, Faculty of Science, University of Technology, Sydney, 2Centre for Health Technologies, Faculty of Engineering and Information Technology, University of Technology, Sydney, 3The Sydney Head and Neck Cancer Institute, Sydney Cancer Centre, Royal Prince Alfred Hospital

Summary
Automatic Translation
June 19th, 2014

This protocol describes a method for extracting small RNAs from human serum. We have used this method to isolate microRNAs from cancer serum for use in DNA arrays and also singleplex quantitative PCR. The protocol utilizes phenol and guanidinium thiocyanate reagents with modifications to yield high quality RNA.

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