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Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
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Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

DOI:
10.3791/51446-v

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14:43 min

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August 27, 2014

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, , , ,
1LENS – European Laboratory for Non-linear Spectroscopy,University of Florence, 2Chemistry Research Laboratory,University of Oxford, 3Department of Biology,University of Florence, 4Department of Physics and Astronomy,University of Florence, 5National Institute of Optics-National Research Council, Italy, 6International Center of Computational Neurophotonics

Chapters

  • 00:05Title
  • 02:39Optical Tweezers Setup with Nanometer Stability
  • 04:40Microfluidics
  • 08:36Combined Optical Trapping and Single-molecule Fluorescence Imaging Experiments
  • 11:50Results: Detection of Single Protein Molecules Interacting with a Single DNA Molecule
  • 12:56Conclusion

Summary

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Here we describe the instrumentation and methods for detecting single fluorescently-labeled protein molecules interacting with a single DNA molecule suspended between two optically trapped microspheres.

Tags

Protein-DNA InteractionOptical TweezersSingle-molecule FluorescenceLactose RepressorDNA Target Sequence1D DiffusionMolecular MotorsSingle-molecule ManipulationSingle-molecule Imaging

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