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Journal / Biology / Die Reinigung des Cystic Fibrosis Transmembran…
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Biology

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JoVE Journal Biology
Die Reinigung des Cystic Fibrosis Transmembran Regulator-Protein in Ausgedrückt
Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
Journal (Biology)
Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin
Journal (Biology)
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Die Reinigung des Cystic Fibrosis Transmembran Regulator-Protein in Ausgedrückt

Article DOI: 10.3791/51447-v • 15:12 min • May 10th, 2014
May 10th, 2014 •
1, 1, 1, 1
1Faculty of Life Sciences, University of Manchester

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Die heterologe Expression und Reinigung des Mukoviszidose Transmembran Regulator (CFTR) sind bedeutende Herausforderungen und limitierende Faktoren bei der Entwicklung von Arzneimitteltherapien für Mukoviszidose. Dieses Protokoll beschreibt zwei Verfahren zur Isolierung von Milligramm-Mengen von CFTR für funktionelle und strukturelle Untersuchungen.

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Biochemie Membran-Protein zystische Fibrose CFTR ABCC7 Proteinreinigung Cystic Fibrosis Foundation grün fluoreszierende Protein
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