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JoVE Journal
Neuroscience
Determination of the Spontaneous Locomotor Activity in Drosophila melanogaster
Determination of the Spontaneous Locomotor Activity in Drosophila melanogaster
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Determination of the Spontaneous Locomotor Activity in Drosophila melanogaster

Determination of the Spontaneous Locomotor Activity in Drosophila melanogaster

Full Text
13,259 Views
08:06 min
April 10, 2014

DOI: 10.3791/51449-v

Jared K. Woods1, Suzanne Kowalski1, Blanka Rogina1

1Genetics and Developmental Biology, School of Medicine,University of Connecticut Health Center

Drosophila melanogaster are useful in studying genetic or environmental manipulations that affect behaviors such as spontaneous locomotor activity.  Here we describe a protocol that utilizes monitors with infrared beams and data analysis software to quantify spontaneous locomotor activity. 

The overall goal of this procedure is to quantify spontaneous locomotor activity of fruit flies. This is accomplished by first collecting and aging experimental flies. The second step is to set up population monitors in a temperature controlled incubator and download the appropriate software.

Next, the flies are transferred to glass files and then placed inside population monitors where the activity monitors are run. The final step is to analyze the data. Ultimately, population monitors are used to show differences in spontaneous physical activity of flies.

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