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ניטור Juxtacellular והלוקליזציה של Single נוירונים בתוך מבנים מוחיים תת-קליפת המוח של התראה, חולדות-מרוסן ראש
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Juxtacellular Monitoring and Localization of Single Neurons within Sub-cortical Brain Structures of Alert, Head-restrained Rats
DOI:

08:41 min

April 27, 2015

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Chapters

  • 00:05Title
  • 01:18Implanting the Recording Chamber and Head-restraint Mechanism
  • 05:01Juxtacellular Monitoring of Neuronal Units
  • 07:34Results: Neuronal Units in Ventral Posterior Medial Thalamus Encode the Phase of Vibrissa Movement During Self-generated Whisking
  • 08:13Conclusion

Summary

Automatic Translation

This protocol describes the design and surgical implantation of a head-restraining mechanism to monitor neuronal activity in sub-cortical brain structures in alert rats. It delineates procedures to isolate single neurons in the juxtacellular configuration and to efficiently identify their anatomical locations.

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