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A Microfluidic Technique to Probe Cell Deformability
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Bioengineering
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JoVE Journal Bioengineering
A Microfluidic Technique to Probe Cell Deformability

A Microfluidic Technique to Probe Cell Deformability

DOI:
10.3791/51474-v

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09:47 min

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September 03, 2014

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, , ,
1Department of Integrative Biology and Physiology,University of California, Los Angeles, 2Department of Aerospace and Mechanical Engineering,University of Notre Dame, 3Molecular Imaging Center,University of Southern California

Chapters

  • 00:05Title
  • 01:25Supplies and Preparation
  • 03:33Microfluidic Device Fabrication
  • 05:36Deforming Cells Through Constricted Channels
  • 06:52Data Analysis
  • 08:04Results: Representative Transit Time Measurements for Different Cells Types
  • 09:12Conclusion

Summary

Automatic Translation

Automatic Translation

We demonstrate a microfluidics-based assay to measure the timescale for cells to transit through a sequence of micron-scale constrictions.

Tags

Microfluidic DeviceCell DeformabilityCell DeformationPressure-driven FlowImage AnalysisHL-60 CellsAll-trans Retinoic AcidNeutrophil-typeViscoelastic PropertiesMolecular Origins

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