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Bioengineering

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JoVE Journal Bioengineering
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Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Article DOI: 10.3791/51501 • 07:56 min • May 8th, 2014
May 8th, 2014 •
1, 1, 2, 3, 3, 3,4, 1,2
1Department of Electrical and Computer Engineering, University of Minnesota, 2Department of Biomedical Engineering, University of Minnesota, 3Department of Neurology, Mayo Clinic College of Medicine, 4Department of Immunology, Mayo Clinic College of Medicine

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Summary
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Supported lipid bilayers and natural membrane particles are convenient systems that can approximate the properties of cell membranes and be incorporated in a variety of analytical strategies. Here we demonstrate a method for preparing microarrays composed of supported lipid bilayer-coated SiO2 beads, phospholipid vesicles or natural membrane particles.

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Biomembrane Microarray Squeegee-based Assembly Method Lipid Bilayer Membranes Plasma Membranes Subcellular Organelles Heterogeneous Mixtures Lipids Membrane-bound Proteins Carbohydrates Decouple Biophysical Properties Biochemical Properties Model Systems Giant Vesicles Liposomes Supported Lipid Bilayers (SLBs) Sensing Applications Cell-cell Interactions Submicron-diameter SiO2 Beads Spherical SLBs (SSLBs) Micro-fabricated Submicron-diameter Microwells Preparation Technique Substrate Surface Cleaning Targeting Ligands Occupied Microwells Buffer SSLB Arrays
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