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JoVE Journal
Bioengineering

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Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds
 

Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds

Article DOI: 10.3791/51517
August 16th, 2014

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Summary August 16th, 2014

This protocol combines electrospinning and microspheres to develop tissue engineered scaffolds to direct neurons. Nerve growth factor was encapsulated within PLGA microspheres and electrospun into Hyaluronic Acid (HA) fibrous scaffolds. The protein bioactivity was tested by seeding the scaffolds with primary chick Dorsal Root Ganglia and culturing for 4-6 days.

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