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Two-Photon in vivo Imaging of Dendritic Spines in the Mouse Cortex Using a Thinned-skull Preparation
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Chapters
- 00:05Title
- 00:56Surgery
- 01:46Thinned-skull Preparation
- 03:27Immobilization
- 04:45Imaging
- 06:33Recovery
- 07:14Re-imaging
- 08:22Results: Transcranial Two-Photon Imaging Showing Dynamics of Dendritic Spines
- 09:31Conclusion
Time-lapse imaging in the living animal provides valuable information on structural reorganization in the intact brain. Here, we introduce a thinned-skull preparation that allows transcranial imaging of fluorescently labeled synaptic structures in the living mouse cortex by two-photon microscopy.
