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An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis
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JoVE Journal Biology
An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis

An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis

DOI:
10.3791/51530-v

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09:33 min

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June 19, 2014

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, ,
1Institute of Plant Biology and Zürich-Basel Plant Science Center,University of Zürich, 2Institut de Recherche pour le Développement (UMR 232), Centre National de la Recherche Scientifique (ERL 5300),Université de Montpellier II

Chapters

  • 00:05Title
  • 01:15Tissue Fixation, Dissection and Embedding
  • 03:21Tissue Processing
  • 05:41Immunostaining and Imaging
  • 07:32High-Resolution 3D Quantitative Imaging in Ovules
  • 08:40Conclusion

Summary

Automatic Translation

Automatic Translation

We provide here an efficient and reliable protocol for immunostaining, Fluorescence in situ Hybridization, DNA staining followed by quantitative, high-resolution imaging in whole-mount Arabidopsis thaliana ovules. This method was successfully used to analyze chromatin modifications and nuclear architecture.

Tags

Keywords: ArabidopsisOvuleSingle-cell AnalysisChromatin ModificationNuclear ArchitectureImmunodetectionFluorescence In Situ Hybridization (FISH)Confocal Laser Scanning Microscopy (CLSM)

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