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Screening siRNA para identificar ubiquitina y reguladores del sistema ubiquitina-como de las vías biológicas en células de mamífero en cultivo
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Screening siRNA para identificar ubiquitina y reguladores del sistema ubiquitina-como de las vías biológicas en células de mamífero en cultivo

Article DOI: 10.3791/51572-v • 10:43 min • May 24th, 2014
May 24th, 2014 •
1, 1, 1, 2, 1,2
1MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, 2Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee

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Aquí se describe una metodología para llevar a cabo un siRNA dirigidos pantalla "ubiquitome" para identificar nuevos ubiquitina y reguladores-ubiquitina de la respuesta celular mediada HIF1A a la hipoxia. Esto puede ser adaptado a cualquier vía biológica, donde una lectura robusta de la actividad del indicador está disponible.

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Bioquímica screening siRNA ubiquitina UBL ubiquitome hipoxia HIF1A de alto rendimiento las células de mamíferos el reportero de la luciferasa
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