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Tissue Triage and Freezing for Models of Skeletal Muscle Disease

Article DOI: 10.3791/51586 • 05:58 min • July 15th, 2014
July 15th, 2014 •
1, 2, 3, 4, 5, 5, 1,6, 7, 8, 9, 5, 1
1Division of Pediatric Pathology, Department of Pathology and Laboratory Medicine, Medical College of Wisconsin, 2Department of Physiology and Cell Biology, The Ohio State University, 3Department of Human Nutrition, Foods and Exercise, Virginia Tech, 4Division of Biomedical Informatics, Department of Biostatistics, Department of Computer Science, University of Kentucky, 5Division of Genetics and Genomics, The Manton Center for Orphan Disease Research, Boston Children's Hospital, Harvard Medical School, 6Cure Congenital Muscular Dystrophy, 7Joshua Frase Foundation, 8Department of Rehabilitation Medicine, University of Washington, 9Department of Physiology, University of Arizona

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Summary
Automatic Translation

The analysis of skeletal muscle tissues to determine structural, functional, and biochemical properties is greatly facilitated by appropriate preparation. This protocol describes appropriate methods to prepare skeletal muscle tissue for a broad range of phenotyping studies.

Tags

Tissue Triage Freezing Skeletal Muscle Disease Functional Evaluations Cellular Evaluations Molecular Evaluations Pathological Evaluations Congenital Muscle Disorders Frozen Muscle Fixed Muscle Freezing Artifacts Histological Examination Isopentane Cooling Liquid Nitrogen Preservation Genetic Studies Protein Expression Studies Triage Of Tissue Single Fiber Functional Studies Myoblast Cell Culture
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