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Journal / Immunology and Infection / Majör Histo-uyum Kompleks sınıf II Dextramers…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Majör Histo-uyum Kompleks sınıf II Dextramers kullanarak Beyin ve Heart reaktif CD4 T hücrelerinin in situ tespiti
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Majör Histo-uyum Kompleks sınıf II Dextramers kullanarak Beyin ve Heart reaktif CD4 T hücrelerinin in situ tespiti

Article DOI: 10.3791/51679-v • 13:10 min • August 1st, 2014
August 1st, 2014 •
1, 1, 1, 2, 3, 2, 1
1School of Veterinary Medicine and Biomedical Sciences, University of Nebraska, Lincoln, 2Center for Biotechnology, University of Nebraska, Lincoln, 3Nebraska Center for Virology and School of Biological Sciences, University of Nebraska, Lincoln

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MHC kompleksi sınıf II dextramers ile direkt boyama ile beyin ve kalp kendinden reaktif CD4 T hücreleri algılamak için protokol bu raporda tarif edilmiştir. Kapsamlı bir analiz için in situ antijene özgü CD4 + T hücrelerinin frekansları numaralandırmak için güvenilir bir yöntem de planlanmaktadır.

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İmmünoloji Sayı 90 dextramers; MHC sınıf II;
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