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Fundamental Technical Elements of Freeze-fracture/Freeze-etch in Biological Electron Microscopy
 

Fundamental Technical Elements of Freeze-fracture/Freeze-etch in Biological Electron Microscopy

Article DOI: 10.3791/51694
September 11th, 2014

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Summary September 11th, 2014

Basic techniques and refinements of freeze-fracture processing of biological specimens and nanomaterials for examination by transmission electron microscopy are described. This technique is a preferred method for revealing ultrastructural features and specializations of biological membranes and for obtaining ultrastructural level dimensional and spatial data in materials sciences and nanotechnology products.

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