In order to understand the cellular and molecular mechanisms underlying neotissue formation and stenosis development in tissue engineered heart valves, a murine model of heterotopic heart valve transplantation was developed. A pulmonary heart valve was transplanted to recipient using the heterotopic heart transplantation technique.
Lee, Y., Yi, T., James, I., Tara, S., Stuber, A. J., Shah, K. V., Lee, A. Y., Sugiura, T., Hibino, N., Shinoka, T., Breuer, C. K. Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation. J. Vis. Exp. (89), e51695, doi:10.3791/51695 (2014).