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Real-Time Impedance-based Cell Analyzer as a Tool to Delineate Molecular Pathways Involved in Neurotoxicity and Neuroprotection in a Neuronal Cell Line
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Neuroscience
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JoVE Journal Neuroscience
Real-Time Impedance-based Cell Analyzer as a Tool to Delineate Molecular Pathways Involved in Neurotoxicity and Neuroprotection in a Neuronal Cell Line

Real-Time Impedance-based Cell Analyzer as a Tool to Delineate Molecular Pathways Involved in Neurotoxicity and Neuroprotection in a Neuronal Cell Line

DOI:
10.3791/51748-v

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08:23 min

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August 09, 2014

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, ,
1University Clinics for Child and Adolescent Psychiatry,University of Zürich

Chapters

  • 00:05Title
  • 01:39Plating and Proliferation of SK-N-SH Cells
  • 03:59Serum Deprivation
  • 05:04Data Analysis
  • 06:11Results: Representative Neurotoxicity/Neuroprotection Data
  • 07:53Conclusion

Summary

Automatic Translation

Automatic Translation

Improved in vitro neurotoxicity assays would aid the identification of new neuroprotective compounds. The utility of a real-time impedance-based cell analyzer to determine cytotoxicity and cytoprotection in neuronal cell lines and to delineate the involvement of second messenger pathways, thus gaining insight in the mechanism of neuroprotection is presented.

Tags

Keywords: Real-time Impedance-based Cell AnalysisNeuronal Cell LineNeuroprotectionNeurotoxicity5-HT2A Receptor AgonistsSecond Messenger PathwaysCell ProliferationCell IndexLabel-free Assessment

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