High-throughput Assay to Phenotype <em>Salmonella enterica </em>Typhimurium Association, Invasion, and Replication in Macrophages

Jing Wu; Roberta Pugh; Richard C. Laughlin; Helene Andrews-Polymenis; Michael McClelland; Andreas J. B&#228;umler; L. Garry Adams

doi:10.3791/51759

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High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

12,560 Views

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11:10 min

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August 11, 2014

DOI:

10.3791/51759-v

DOI:

10.3791/51759-v

•

11:10 min

August 11, 2014

•

6 Views

Jing Wu¹, Roberta Pugh¹, Richard C. Laughlin¹, Helene Andrews-Polymenis², Michael McClelland³, Andreas J. Bäumler⁴, L. Garry Adams¹

¹Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences,Texas A&M University, ²Department of Microbial and Molecular Pathogenesis, College of Medicine,Texas A&M University System Health Science Center, ³University of California, Irvine, ⁴Department of Medical Microbiology & Immunology, School of Medicine,University of California, Davis

Summary

A high-throughput assay to in vitro phenotype Salmonella or other bacterial association, invasion, and replication in phagocytic cells with high-throughput capacity was developed. The method was employed to evaluate Salmonella gene knockout mutant strains for their involvements in host-pathogen interactions.