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JoVE Journal
Biology

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A Video Protocol of Retroviral Infection in Primary Intestinal Organoid Culture
 

A Video Protocol of Retroviral Infection in Primary Intestinal Organoid Culture

Article DOI: 10.3791/51765
August 11th, 2014

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Summary August 11th, 2014

This protocol explains primary Lgr5-positve organoid culture and the subsequent performance of retroviral transduction. This enables Cre-inducible overexpression or knockdown of the delivered transgene and allows functional studies to be carried out in the novel in vitro organotypic model system.

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