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En video-protokollet av retroviral infektion i Primär Intestinal organoid kultur
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En video-protokollet av retroviral infektion i Primär Intestinal organoid kultur

Article DOI: 10.3791/51765-v • 09:18 min • August 11th, 2014
August 11th, 2014 •
1,2, 1,2, 2, 1,2
1Department of Genetics, University of Cambridge, 2Wellcome Trust - Medical Research Council Stem Cell Institute, University of Cambridge

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Detta protokoll förklarar primära Lgr5-positve organoid kulturen och den efterföljande prestanda retroviral transduktion. Detta gör att Cre-inducerbart överuttryck eller knockdown av den levererade transgenen och tillåter funktionella studier som skall utföras i romanen in vitro organotypisk modellsystem.

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Genetik Retrovirus Lentivirus organoid kultur Lgr5 tarm 3R
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