RNA Isolation from Mouse Pancreas: A Ribonuclease-rich Tissue

The overall goal of this procedure is to isolate RNA with high integrity from the Ribonuclease ridge mouse pancreas. This is accomplished by quickly removing the pancreas from the carcass of a euthanized mouse. Next, the pancreas has submerged in lysis reagent, and the tissue is homogenized.

Following centrifugation, the RNA is isolated from the supernatant using standard protocols and RNAs inhibitor solution is added to the final RNA solution. The final step is to carefully clean the homogenizer blades by removing any chunks of tissue caught in the blades, and then performing a series of ethanol and water washes. Ultimately, this procedure allows for the isolation of RNA for mouse pancreas with an RNA integrity number greater than seven to be used for routine gene expression analysis.