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Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins
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Bioengineering
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JoVE Journal Bioengineering
Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins

DOI:
10.3791/51789-v

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10:05 min

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August 07, 2014

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, , , ,
1Center for Applied Proteomics and Molecular Medicine,George Mason University, 2Ceres Nanosciences

Chapters

  • 00:05Title
  • 01:47Nanoparticle Processing of Serum Samples
  • 03:44Nanoparticle Processing of Urine Samples
  • 08:07Results: Nanoparticle Capture and Concentration of Proteins from Plasma or Urine
  • 09:31Conclusion

Summary

Automatic Translation

Automatic Translation

Several pathological biomarkers cannot be easily detected by current techniques because of their low concentration in biological fluids, the presence of degrading enzymes, and large amounts of high molecular weight proteins. Chemically functionalized hydrogel nanoparticles can harvest, preserve and concentrate low abundance proteins enabling the detection of previously undetectable biomarkers.

Tags

Hydrogel NanoparticlePlasmaUrineLow Abundance ProteinsBiomarker DiscoveryMass SpectrometryImmunoassayPoly(N-isopropylacrylamide)NIPAmProtein CaptureProtein ConcentrationDetection Limit

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