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微流体为基础的电化学生物芯片的无标记的DNA杂交分析

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

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微流体为基础的电化学生物芯片的无标记的DNA杂交分析

DOI:

10.3791/51797-v

•

14:53 min

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September 10, 2014

•

Hadar Ben-Yoav, Peter H. Dykstra, Tanya Gordonov, William E. Bentley, Reza Ghodssi

¹MEMS Sensors and Actuators Laboratory (MSAL), Department of Electrical and Computer Engineering, Institute for Systems Research,University of Maryland, ²Institute for Bioscience and Biotechnology Research, Fischell Department of Bioengineering,University of Maryland

Chapters

00:05Title
02:06Microfabrication of the Electrochemical Chip
06:36Fabrication of the PDMS Chip
08:52Assembly and Preparation of the Microfluidic Arrayed Electrochemical Device
10:44DNA Hybridization Analysis
12:14Validating Electrochemical Activity of the Biochip and Detection of Hybridization Events
13:55Conclusion

Summary

Automatic Translation

我们提出了一个微流体为基础的电化学生物芯片的DNA杂交检测。以下的ssDNA探针官能化，特异性，灵敏度和检测极限进行了研究具有互补和非互补ssDNA的目标。结果表明在电化学系统中的DNA杂交事件的影响，以3.8纳米的检测极限。

Tags

Microfluidic Electrochemical Biochip Label-free DNA Hybridization Analysis Miniaturization Point-of-care Electrochemical Impedance Spectroscopy Charge Transfer Diffusional Resistance Biosensor SsDNA Detection

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