Cell Suspension
Cell Suspension - Related Videos
11:17
Generation of Single-Cell Suspensions from Mouse Neural Tissue
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Cited by 16 •
2009
Dissociating cells from specific tissue types requires specific parameters for tissue aggitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS Dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on nerual tissue is explained.
13:55
Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions
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Cited by 40 •
2013
Vacuolar and cytosolic pH can be measured in live yeast (S. cerevisiae) cells using ratiometric fluorescent dyes localized to specific cellular compartments. We describe procedures for measuring vacuolar pH with BCECF-AM, which localizes to the vacuole in yeast, and cytosolic pH with a cytosolic ratiometric pH-sensitive GFP (yeast pHluorin).
10:17
The Tail Suspension Test
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Cited by 534 •
2012
The tail-suspension test is validated as an experimental procedure to assess antidepressant efficacy of drug treatments in mice. Mice are suspended by their tails for six minutes and escape-related behaviors are assessed. We describe procedures used in conducting the tail suspension test.
15:33
Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions
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Cited by 20 •
2013
A novel protocol for the mechanical preparation of testicular cell suspensions from rodent material, avoiding enzymes and detergents, is described. The method is very simple, fast, reproducible, and renders good quality cell suspensions, which are suitable for flow sorting and RNA extraction.
11:08
Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry
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Cited by 4 •
2017
This article describes spectral cytometry, a new approach in flow cytometry that uses the shapes of emission spectra to distinguish fluorochromes. An algorithm replaces compensations and can treat auto-fluorescence as an independent parameter. This new approach allows for the proper analysis of cells isolated from solid organs.
FAQs
Gentle agitation helps limit settling by keeping cells or small aggregates distributed through the liquid medium. However, the movement must remain controlled because excessive agitation can create shear stress that harms cells. Successful maintenance therefore depends on balancing adequate mixing with conditions that preserve cell viability and support continued growth.
These conditions provide the chemical and physical environment required for cells to remain viable and continue growing. Nutrient availability supports cellular activity, while suitable pH and temperature help maintain stable culture conditions. If these factors are not controlled, the suspension may become unsuitable for expansion, analysis, or later transfer.
Suspension cultures may begin with cells that naturally live freely in liquid or with cells adapted from an adherent growth format. This origin matters because the culture must be maintained in a form that allows cells to remain dispersed or present as small aggregates. The distinction provides useful context when interpreting growth and handling requirements.
A suitable liquid medium, appropriate nutrients, controlled pH and temperature, and gentle agitation form the central maintenance conditions. Together, these factors support cell growth while limiting settling and excessive mechanical stress. Maintaining this balance allows the preparation to remain useful for subsequent transfer, expansion, microscopy, or other biological measurements.
A cell suspension can support microscopy, flow cytometry, viability measurements, and biochemical assays. These approaches provide complementary information about the cells, including visual characteristics, measurable population properties, survival, and biochemical activity. Using a dispersed preparation also makes the cells accessible for analysis under controlled experimental conditions.
The approach is useful when researchers need to expand cells, study cellular behavior, produce biological materials, or scale cultures for research and industrial applications. Its value extends beyond routine maintenance because the same preparation can support controlled analysis, experimental transfer, and larger-scale cultivation within biology and biotechnology workflows.