16,184 Views
•
09:51 min
•
January 29, 2015
DOI:
Summary
Automatically generated
We describe procedures for labeling and genotyping newborn mice and generating primary neuronal cultures from them. The genotyping is rapid, efficient and reliable, and allows for automated nucleic-acid extraction. This is especially useful for neonatally lethal mice and their cultures that require prior completion of genotyping.
Related Videos
08:24
In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
Related Videos
17659 Views
16:01
Multi-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures
Related Videos
26345 Views
16:33
Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)
Related Videos
35533 Views
08:22
Calcium Phosphate Transfection of Primary Hippocampal Neurons
Related Videos
25104 Views
11:58
Primary Culture of Mouse Dopaminergic Neurons
Related Videos
37561 Views
08:45
Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains
Related Videos
13852 Views
10:55
Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations
Related Videos
8528 Views
09:32
Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes
Related Videos
13497 Views
08:09
Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum
Related Videos
19583 Views
05:52
Multi-Fiber Photometry to Record Neural Activity in Freely-Moving Animals
Related Videos
35516 Views
Read Article
Cite this Article
Koh, J., Iwabuchi, S., Huang, Z., Harata, N. C. Rapid Genotyping of Animals Followed by Establishing Primary Cultures of Brain Neurons. J. Vis. Exp. (95), e51879, doi:10.3791/51879 (2015).
Copy