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Rapid Genotyping of Animals Followed by Establishing Primary Cultures of Brain Neurons
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Chapters
- 00:05Title
- 01:33Fast PCR Genotyping of Newborn Mice
- 02:56Primary Culture of Mouse Brain Neurons
- 08:01Results: Representative Genotyping of Primary Neuronal Culture
- 09:17Conclusion
We describe procedures for labeling and genotyping newborn mice and generating primary neuronal cultures from them. The genotyping is rapid, efficient and reliable, and allows for automated nucleic-acid extraction. This is especially useful for neonatally lethal mice and their cultures that require prior completion of genotyping.
