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Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria
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Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria

Article DOI: 10.3791/51887 • 11:53 min • September 18th, 2014
September 18th, 2014 •
1, 2, 2
1Department of Biochemistry, Université de Montréal, CRCHUM, 2Department of Neurosciences, Université de Montréal, CRCHUM

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Described here is a method to detect and quantify mitochondrial outer membrane proteins by immunolabeling of mitochondria isolated from rodent tissue and analysis by flow cytometry. This method can be extended to assess functional aspects of mitochondrial subpopulations.

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Immunodetection Outer Membrane Proteins Flow Cytometry Isolated Mitochondria Animal Tissues Mitochondrial Physiology Pathophysiology Immunolabeling Rodent Tissue Rapid Enrichment Myelin Removal Antibody Labeling Fluorescently Conjugated Secondary Antibody Flow Cytometry Analysis Mitochondrial Purity Mitochondrial Specific Dye Immunolabeled Protein Quantification High-throughput Analysis Novel Proteins At Mitochondrial Surface Protein Mislocalization During Pathology Fluorescent Indicator Dyes Central Nervous System Mitochondria (brain And Spinal Cord) Liver Mitochondria
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