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Biology

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Generation of Transgenic Hydra by Embryo Microinjection
 

Generation of Transgenic Hydra by Embryo Microinjection

Article DOI: 10.3791/51888
September 11th, 2014

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Summary September 11th, 2014

Stably transgenic Hydra are made by microinjection of plasmid DNA into embryos followed by random genomic integration and asexual propagation to establish a uniform line. Transgenic Hydra are used to track cell movements, overexpress genes, study promoter function, or knock down gene expression using RNAi.

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