A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.
Summary September 16th, 2014
We describe here an improved Luminescence Resonance Energy Transfer (LRET) method where we introduce a protease cleavage site between the donor and acceptor fluorophore sites. This modification allows us to obtain specific LRET signals arising from membrane proteins of interest, allowing for the study of membrane proteins without protein purification.