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Real-time Imaging af axonal Transport af Quantum Dot-mærket BDNF i Primary neuroner
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
DOI:

10:53 min

September 15, 2014

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Chapters

  • 00:05Title
  • 01:32Preparation of Microfluidic Chambers
  • 03:26Dissection of Neuronal Culture and Plating on Chambers
  • 05:41Axonal Transport of Quantum Dot-labeled Brain-derived Neurotrophic Factor (QD-BDNF)
  • 07:43Results: Live Imaging of Axonal Transport of QD-BDNF in Hippocampal Neurons
  • 10:27Conclusion

Summary

Automatic Translation

Axonal transport af BDNF, en neurotrofisk faktor, er afgørende for overlevelse og funktion af flere neuronale populationer. Visse degenerative sygdomme er kendetegnet ved forstyrrelser af axonal struktur og funktion. Vi demonstrerede de anvendte teknikker til at undersøge levende handel med QD-BDNF i mikrofluide kamre anvender primære neuroner.

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