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JoVE Journal Biology
Methylnitrosourea (MNU) -inducerede nethindedegeneration og Regeneration i zebrafisk
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Methylnitrosourea (MNU) -inducerede nethindedegeneration og Regeneration i zebrafisk: Histologisk og funktionelle egenskaber

Article DOI: 10.3791/51909-v • 08:53 min • October 20th, 2014
October 20th, 2014 •
1, 1,2, 1, 3, 3, 1
1Department of Ophthalmology, Inselspital, University of Bern, 2Department of Ophthalmology, University Hospital of Basel, 3Department of Biology, University of Fribourg

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Heri vi demonstrere kvantificering af retinal de- og regenerering og dens indvirkning på visuel funktion ved anvendelse af N-methyl-N -nitrosourea i den voksne zebrafisk. Tab af synsstyrke og nedsat fotoreceptor numre blev efterfulgt af proliferation i den indre nukleare lag. Komplet morfologiske og funktionelle regenerering forekom 30 dage efter den indledende behandling.

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Cellebiologi , nethinde degeneration fotoreceptorer Müller celler regenerering zebrafisk visuel funktion
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